Journal: Nature communications

Article Title: Fibromodulin selectively accelerates myofibroblast apoptosis in cutaneous wounds by enhancing interleukin 1β signaling.

doi: 10.1038/s41467-025-58906-z

Figure Lengend Snippet: Fig. 8 | Fibromodulin (FMOD) binds to the interleukin (IL)1β-interleukin 1 receptor type 1 (IL1R1)-interleukin-1 receptor accessory protein (IL1RAP) ternary complex. a Image depicting the pull-down assay conducted with recom- binant human His-tagged FMOD, which was incubated in vitro with recombinant IL1β. b Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1R1. c Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with recombinant IL1RAP. d Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with IL1β-IL1R1-IL1RAP ternary complex (Com.) in an acellular system. e Image depicting the pull-down assay conducted with recombinant human His-tagged FMOD, which was incubated in vitro with the whole membrane protein

Article Snippet: Antibodies used for pulldown assay include rabbit anti-fibromodulin antibody (H50) (sc-33772, Santa Cruz Biotechnology, Santa Cruz, CA; 1:500 dilution), goat antihuman IL-1 beta/IL-1F2 antibody (AF-201-NA, R&D systems; 0.1 μg/mL), rabbit anti-IL1 receptor I/IL-1R-1 antibody (ab154524; Abcam; 1:1000 dilution), mouse-anti IL-1RAcP (D5) (sc-376872, Santa Cruz Biotechnology; 1:100 dilution), donkey anti-goat IgG (H + L) secondary antibody, HRP (A15999, Thermo Fisher Scientific; 1:5000 dilution), donkey anti-rabbit IgG (H+ L) secondary antibody, HRP (A16023, Thermo Fisher Scientific; 1:5000 dilution), and donkey anti-mouse IgG (H + L) cross-adsorbed secondary antibody, HRP (SA1-100, Thermo Fisher Scientific; 1: 2000 dilution).

Techniques: Pull Down Assay, Incubation, In Vitro, Recombinant, Membrane

Journal: Nature communications

Article Title: Fibromodulin selectively accelerates myofibroblast apoptosis in cutaneous wounds by enhancing interleukin 1β signaling.

doi: 10.1038/s41467-025-58906-z

Figure Lengend Snippet: Fig. 10 | Fibromodulin (FMOD) promotes the direct binding of interleukin 1 receptor type 1 (IL1R1) and interleukin-1 receptor accessory protein (IL1RAP) on the surface of BJ-myofibroblasts. All cells were subjected to serum starvation prior to treatment. a Representative images of in situ IL1R1:IL1RAP-proximity liga- tion assay (PLA) staining on attached BJ-myofibroblasts. Phalloindin was used to stain F-actin, while DAPI was used for nuclei staining. b–g Quantification of IL1R1:IL1RAP-PLA+ signal per cell at 1- (b), 6- (c), 12- (d), 24- (e), 48- (f), and 72-h (g)

Article Snippet: Antibodies used for pulldown assay include rabbit anti-fibromodulin antibody (H50) (sc-33772, Santa Cruz Biotechnology, Santa Cruz, CA; 1:500 dilution), goat antihuman IL-1 beta/IL-1F2 antibody (AF-201-NA, R&D systems; 0.1 μg/mL), rabbit anti-IL1 receptor I/IL-1R-1 antibody (ab154524; Abcam; 1:1000 dilution), mouse-anti IL-1RAcP (D5) (sc-376872, Santa Cruz Biotechnology; 1:100 dilution), donkey anti-goat IgG (H + L) secondary antibody, HRP (A15999, Thermo Fisher Scientific; 1:5000 dilution), donkey anti-rabbit IgG (H+ L) secondary antibody, HRP (A16023, Thermo Fisher Scientific; 1:5000 dilution), and donkey anti-mouse IgG (H + L) cross-adsorbed secondary antibody, HRP (SA1-100, Thermo Fisher Scientific; 1: 2000 dilution).

Techniques: Binding Assay, In Situ, Staining

Journal: Structure (London, England : 1993)

Article Title: Functional Relevance of IL-1 Receptor Inter-domain Flexibility for Cytokine Binding and Signaling

doi: 10.1016/j.str.2019.05.011

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit Anti-IL-1RAcP Antibody , Rockland , Cat# 600–401-BV3; RRID:AB_2613201.

Techniques: Recombinant, Plasmid Preparation, Software

Journal: Methods in Molecular Biology

Article Title: Allergy

doi: 10.1007/978-1-4939-9591-2

Figure Lengend Snippet: Fig. 1 Different types of immunoelectrophoresis of cat hair extract (Fel d) using a polyclonal rabbit antibody raised against the same extract (a Fel d). (a) CIE of Fel d extract, (b) FRIE following a purification (immunoab- sorption) of the major allergen Fel d 1, (c) CIE of purified Fel d 1 against a Fel d, (d) CLIE with Fel d 1 in inter- mediate gel for identification of the allergen, (e) TCIE with Fel d extract and Fel d 1 showing a double peak, (f) CIIE with monospecific Fel d 1 in intermediate gel

Article Snippet: Detection: Biotinylated goat anti-human affinity-purified polyclonal IL-RAcP/IL-1R3 (R&D Systems).

Techniques: Immunoelectrophoresis, Purification

Journal: Reproduction

Article Title: Stable inhibition of interleukin 1 receptor type II in Ishikawa cells augments secretion of matrix metalloproteinases: possible role in endometriosis pathophysiology

doi: 10.1530/rep-06-0377

Figure Lengend Snippet: Figure 2 Immunofluorescence analysis of IL1R1, IL1R2, and IL1RAcP expression in non-transfected Ishikawa cells (NT) and E03, E11, A08, and A17 clones. Note the com- parable intensity of IL1R1 and IL1RAcP immunofluorescent signals in NT, E03, and E11 clones, and the marked decline in IL1R2 immunofluorescent signal in A08 and A17 clones. No immunofluorescence was observedinnegativecontrols(c)in theabsence of primary antibodies (objective !40).

Article Snippet: Membranes were incubated for 3 h at room temperature with a polyclonal goat anti-human IL1R1 (R&D Systems; www.reproduction-online.org 2 mg/ml in blocking solution), a polyclonal goat anti-human IL1R2 (R&D Systems; 2 mg/ml in blocking solution), or a polyclonal goat anti-human IL1RAcP (R&D Systems; 2 mg/ml in blocking solution).

Techniques: Expressing, Transfection, Clone Assay

Journal: Reproduction

Article Title: Stable inhibition of interleukin 1 receptor type II in Ishikawa cells augments secretion of matrix metalloproteinases: possible role in endometriosis pathophysiology

doi: 10.1530/rep-06-0377

Figure Lengend Snippet: Figure 3 Representative Western blot analysis of IL1R1 (A), IL1R2 (B), and IL1RAcP (C) expression in non-transfected Ishikawa cells (NT) and E03, E11, A08, and A17 clones. Equal amounts of proteins were subjected to SDS-PAGE analysis and Western blotting. Recombinant IL1R1, IL1R2, and IL1RAcP proteins were taken as controls (rR). Blotting with anti-tubulin antibody demonstrates equal protein loading. Note the reduced intensity of IL1R2 bands corresponding to either the membrane-bound or the soluble forms of the receptor, when compared with NT cells, and the absence of noticeable change in IL1R1 and IL1RAcP expression; mb, membrane-bound; s, soluble.

Article Snippet: Membranes were incubated for 3 h at room temperature with a polyclonal goat anti-human IL1R1 (R&D Systems; www.reproduction-online.org 2 mg/ml in blocking solution), a polyclonal goat anti-human IL1R2 (R&D Systems; 2 mg/ml in blocking solution), or a polyclonal goat anti-human IL1RAcP (R&D Systems; 2 mg/ml in blocking solution).

Techniques: Western Blot, Expressing, Transfection, Clone Assay, SDS Page, Recombinant, Membrane